Stomata in Leaves

Materials Required

  • Leaves of Mango/Bougainvillea/Petunia
  • Micro slide
  • Forceps
  • Water
  • Cover slip
  • Compound microscope


The Procedure

Real Lab Procedure

  • Take one fresh mango leaf from the petri dish.  
  • Fold the leaf and tear along the lower side of the leaf. 
  • You will notice a thin peel on the edges of the torn portions of the leaf.  
  • Using the forceps, pull out the thin transparent layer from the lower epidermis of the leaf. 
  • Place the epidermis layer on a clean glass slide.  
  • Take few drops of water in a dropper and add 2-3 drops on the glass slide.   
  • Take a cover slip and place it gently on the peel with the aid of a needle. 
  • Remove the extra water using a filter paper. 
  • Place the glass slide on the stage of compound microscope. 
  • Observe the peel under the low power of the microscope and note the different types of cells  
  • Locate the pores in the cells and observe it under high power.  
  • You can repeat the same procedure for observing the cells of upper epidermis.


Simulator Procedure

  • Select the leaves from the drop-down list. 
  • Click on the leaf to tear it. 
  • Drag and drop the forceps towards the leaf to take the epidermal layer of leaf.  
  • Drag and drop the peel towards the glass slide to place it on it. 
  • Drag and drop the dropper towards the glass slide and pour few drops onto it. 
  • Drag and drop the coverslip towards the glass slide to place on it. 
  • Drag and drop the glass slide towards the stage of microscope and place on it. 
  • Click on the lens to view it. 
  • Click on the stomata portion to view it and identify the parts. 
  • Choose the correct answer and drag and drop the correct answer to the corresponding box. 
  • Click on the Reset button to refresh and redo the experiment.