Detection of Albumin in Urine

Sulphosalicylic Acid Test

Materials Required:

Procedure

  • Take 2 ml urine sample in a measuring cylinder from the urine sample bottle.
  • Take a test tube and pour the urine in the test tube.
  • Using a dropper, take some sulphosalicylic acid.
  • Add few drops of sulphosalicylic acid in the test tube containing urine. A whitish color appears in the solution.
  • Using a test tube holder, hold the test tube firmly and heat it gently upon a burner.
  • A whitish or cloudy turbid solution indicates the presence of albumin in the urine sample.

Heller’s Test

Materials Required:

Procedure

  • Take 5 ml concentrated nitric acid in a measuring cylinder from the reagent bottle.
  • Pour the concentrated nitric acid in a test tube from the measuring cylinder.
  • Using a dropper, take some urine from the urine sample bottle.
  • Now incline the test tube and add sample of urine by means of a dropper along the inner side of the test tube so that it forms a layer over the nitric acid.
  • The presence of white ring at the junction of two layers indicates the presence of albumin in the urine.

Simulator Procedure (as performed through the Online Labs)

You can select the type of test from the ‘Select type of test’ drop down list.

Sulphosalicylic acid Test

  • Drag the dropper containing the sulphosalicylic acid towards the test tube to pour the solution into it
  • Click on the knob of the burner to turn it on.
  • Drag the test tube towards the burner to heat it.
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

Heller’s Test

  • Drag the dropper containing the urine sample towards the test tube to pour the sample into it
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

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