Detection of Bile salt in Urine

Smith’s Test

Materials Required:

Procedure:

  • Take 1 ml Smith’s reagent in a measuring cylinder from the reagent bottle.
  • Pour the Smith’s reagent from the measuring cylinder into a test tube.
  • Using a dropper, take some urine from the urine sample bottle.
  • Tilt the test tube and pour the urine along the side of the test tube.
  • A green ring is formed at the junction of two layers indicating the presence of bile salt in urine.

Pettenkofer’s Test

Materials Required:

Procedure

  • Take 2 ml urine in a measuring cylinder from the urine sample bottle.
  • Pour the urine from the measuring cylinder into a test tube..
  • Take some sucrose using a spatula.
  • Put the sucrose into the test tube containing urine.
  • Take 2 ml H2SO4 in a measuring cylinder from the reagent bottle.
  • Tilt the test tube and pour the H2SO4 along the side of the test tube.
  • Appearance of red colour indicates the presence of bile salt in urine.

Simulator Procedure (as performed through the Online Labs)

You can select the type of test from the ‘Select type of test’ drop down list.

Smith’s Test

  • Drag the dropper containing the urine sample towards the test tube to pour the sample slowly along the side of the tube.
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

Pettenkofer’s Test

  • Drag the spatula containing sucrose towards the test tube to put the sucrose into it.
  • Drag the dropper containing the Conc. H2SO4 towards the test tube to pour the sample into it.
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

Cite this Simulator: