Detection of Sugar in Urine

Benedict's Test

Materials required:

Test tube, test tube holder, urine sample, measuring cylinders, Benedict’s solution and burner.

Procedure:

  • Take 2 ml urine sample in a measuring cylinder from the urine sample bottle.
  • Take a test tube and pour the urine sample in it.
  • Take 5 ml Benedict’s reagent in a measuring cylinder.
  • Add Benedict’s reagent to the test tube that contains urine sample.
  • Using a test tube holder, hold the test tube firmly and heat it for 2 minutes on the burner.
  • Keep shaking the test tube while heating.
  • A yellow precipitate appears which indicates the presence of sugar in urine.
  • Depending upon the concentration of sugar in the urine, either green, yellow, or brick red precipitates are formed.

Fehling's test

Materials required

Test tube, test tube holder, urine sample, measuring cylinders, Fehling’s solution A, Fehling’s solution B and burner.

Procedure

  • Take 2 ml urine sample in a measuring cylinder from the urine sample bottle.
  • Take a test tube and pour the urine sample in it.
  • Take 2 ml Fehling’s solution A in a measuring cylinder.
  • Add Fehling’s solution A to the test tube that contains urine sample.
  • Take 2 ml Fehling’s solution B in a measuring cylinder.
  • Add Fehling’s solution B to the test tube that contains urine sample.
  • Using a test tube holder, hold the test tube firmly and heat it gently for 2 minutes on the burner.
  • Keep shaking the test tube while heating.
  • A green precipitate appears which indicates the presence of traces of sugar in urine.
  • Depending upon the concentration of sugar in the urine, either green, yellow or brick red precipitates are formed.

Simulator Procedure (as performed through the Online Labs)

You can select the test from the ‘Select type of test’ drop down list.

Benedict's Test

  • Drag the dropper containing Benedict’s reagent towards the test tube to pour the reagent into it.
  • Click on the knob of the burner to turn it on.
  • Drag the test tube towards the burner to heat it.
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

Fehling’s Test

  • Drag the dropper containing the Fehling’s reagent A towards the test tube to pour the reagent into it.
  • Drag the dropper containing the Fehling’s reagent B towards the test tube to pour the reagent into it.
  • Click on the knob of the burner to turn it on.
  • Drag the test tube towards the burner to heat it.
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

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