Detection of Urea in Urine

Sodium Hypobromite Test

Materials Required:

Procedure:

  • Take 2 ml urine sample in a measuring cylinder from the urine sample bottle.
  • Take a test tube and pour the urine sample into the test tube.
  • Using a dropper, take sodium hypobromite solution.
  • Add few drops of sodium hypobromide solution to the urine sample.
  • Brisk effervescence of nitrogen appears in the test tube which indicates presence of urea in the urine.

Urease test

Materials required:

Procedure:

  • Take 5 ml urine sample in a measuring cylinder from the urine sample bottle.
  • Take a test tube and pour the urine sample into the test tube.
  • Using a dropper, take some phenol red indicator.
  • Add 4-5 drops of phenol red indicator to the test tube.
  • Use a fresh dropper to take few drops of 2% Na2CO3.
  • Add Na2CO3 solution drop by drop until a pink colour develops in the test tube.
  • Take few drops of 1% acetic acid using a fresh dropper.
  • Add 1% acetic acid to the test tube drop by drop until the pink colour disappears.
  • Using a spatula, take some urease powder from the watch glass.
  • Add urease powder to the test tube and shake it well.
  • The pink or red colour appearing in the solution indicates the presence of urea in urine.

Simulator Procedure (as performed through the Online Labs)

You can select the type of test from the ‘Select type of test’ drop down list.

Sodium Hypobromite Test

  • Drag the dropper containing the sodium hypobromite solution towards the test tube to pour the solution into it
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.

Urease Test

  • Drag the dropper containing the Phenol red indicator towards the test tube to pour the indicator solution into it
  • Drag the dropper containing the Na2CO3 towards the test tube to pour the solution into it
  • Drag the dropper containing the Acetic acid towards the test tube to pour the solution into it
  • Drag the spatula containing the urease powder towards the test tube to put the powder into it
  • Drag the test tube to shake.
  • Click on the information icon to see the inference.
  • You can redo the experiment by clicking on the ‘Reset’ button.     

Cite this Simulator: