Materials Required

Real Lab Procedure

Take a blade and a sunflower stem to be sectioned.
Dip the top of the material and the blade in water.
Hold the material to be sectioned horizontally between the thump and the first finger of your left hand.
Hold the blade in the right hand with the edge of the blade facing you and handle at right angles to it.
Cut the sections of the material quickly using the blade.
Transfer the sections in Petri dish containing water.
Using a brush, select a thin uniform and complete section and place it on the glass slide.
Using a dropper, take some safranin solution.
Pour one drop of safranin solution over the section and allow it to stand for two minutes.
Remove excess stain using a filter paper.
Pour a drop of glycerine over the section.
Place a coverslip gently on the section with the aid of a needle.
Remove the excess glycerine using a filter paper.
Repeat the same procedure for the sunflower root, maize stem and maize root and prepare the slides.
Observe each section under the microscope.

To select a particular transverse section of stem or root sample, click on the corresponding icon.
Click and drag the prepared slide of sample and place it on the stage of the compound microscope.
Click on the eye piece of the compound microscope to view the transverse section of the sample.
The power of the lens can be changed by selecting the required lens power from the drop down list ‘Select objective lens’.
You can move the slide to observe each part of the sample by clicking on the directional arrow of the ‘Slide adjustment’.
To repeat the experiment, click the ‘Reset’ button.