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Paper Chromatography
Paper Chromatography
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1)
The R_f value is calculated as
R_f of compound=(Distance travelled by solvent)/(Distance travelled by compound)
R_f of compound=(Distance travelled by compound)/(Distance travelled by solvent)
R_f of compound=(Distance travelled by solvent)/(Distance travelled by solute)
R_f of compound=(Distance travelled by compound)/(Distance travelled by solute)
2)
What are the four primary pigments in a plant leaves?
Chlorophyll a, chlorophyll d, chloroplast and carotenoids
Chlorophyll a, chlorophyll c, chloroplast and carotenoids
Chlorophyll a, chlorophyll d, xanthophylls and carotenoids
Chlorophyll a, chlorophyll b, xanthophylls and carotenoids
3)
What is chromatography?
A simple technique used to separate solvent from solution.
A technique used to separate molecules on the basis of differences of size, shape, mass, charge, solubility and adsorption properties.
A technique used to separate molecules on the basis of colour.
A simple technique used to separate solution of different concentration.
4)
The term chromatography is derived from Greek. Chroma means ___________ and Graphe means_________.
Chroma = pigments and Graphe = writing
Chroma = Solution and Graphe = Colours
Chroma = writing and Graphe = colour
Chroma = colour and Graphe = writing
5)
What is the colour of chlorophyll a?
Yellow
Dark green
Yellowish-green
Orange
6)
How can the different compounds in a mixture in paper chromatography be identified?
Using wavelengths of visible light.
Using Solute concentration.
Using solvent concentration.
Using Rf value.
7)
Given below are the steps in the preparation of a temporary mount of a stained leaf peel. i) Grind the spinach leaves using the mortar and pestle. ii) When solvent rises about 3/4th of the way up the strip, remove the strip carefully and let it dry. iii) Using a thread, hang the filter paper strip into the chromatographic chamber that contains ether acetone solvent. iv) Put a drop of the pigment extract in the middle of the filter paper strip. Which of the following is the correct sequence of steps?
i, iii, iv, ii
i, ii, iii, iv
i, iv, iii, ii
iv, iii, ii, i
