Asexual Reproduction in Amoeba and Yeast

Materials Required

  1. Permanent or prepared slides of;
    • Binary fission in Amoeba
    • Budding in yeast
  2. Compound microscope

Binary fission in Amoeba

Real Lab Procedure

  1. Take the prepared slide of binary fission in amoeba and place it on the stage of the compound microscope.
  2. Adjust the mirror of the microscope so that maximum light is reflected from the mirror on to the slide.
  3. Focus the slide under the microscope’s lens carefully, first under low power and then under high power.
  4. Observe the different phases of binary fission in Amoeba through the lens of the compound microscope.

Observations

  • Amoeba cells are irregular in shape.
  • Karyokinesis can be observed in some stages, with the mother cell elongating and its nucleus dividing into two.
  • Cytokinesis can be observed in some stages, with the division in cytoplasm that forms two daughter cells.

Conclusion

This slide shows the nucleus of Amoeba in the process of division. The body of Amoeba is elongated and has a constriction in the middle. Hence the given slide is the one that shows the asexual reproduction process of binary fission in Amoeba.

Budding In Yeast

Real Lab Procedure

  1. Take the prepared slide of budding in Yeast and place it on the stage of the compound microscope.
  2. Adjust the mirror of the microscope so that maximum light is reflected from the mirror on to the slide.
  3. Focus the slide under the microscope’s lens carefully, first under low power and then under high power.
  4. Observe the different phases of budding in Yeast through the lens of the compound microscope.

Observations

  • We can observe that the yeast cells are spherical or oval in shape.
  • Outgrowths called buds can be seen on the yeast cells. At times a chain of buds are seen on the parent cells.
  • Buds that have separated from the parent cells can also be seen.

Conclusion

The slide shows some yeast cell protuberance or buds. In a few yeast cells a chain of buds can be observed. Hence the given slide is one that shows the asexual reproduction process of budding in Yeast.

Precautions

  • Before placing the slide on the stage of the microscope, ensure the settings of the microscope mirror and diaphragm are correct.
  • Focus the slide under the microscope’s lens carefully, first under low power and then under high power.

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